Journal: Scandinavian journal of immunology

Article Title: Advanced glycation end products upregulate C-reactive protein synthesis by human hepatocytes through stimulation of monocyte IL-6 and IL-1 beta production.

doi: 10.1111/j.1365-3083.2007.02001.x

Figure Lengend Snippet: Figure 1 Representative morphological identification and immunofluo- rescent staining of CK18 and AFP of human fetal hepatocyte (HFH) cultures. HFH were cultured in Ham’s F12 hormonally defined medium for 7 days and the typical structure of HFH was viewed with phase-con- trast microscopy (Image A, original magnification ·100). Images B and C show HFH cultured in Ham’s F12 hormonally defined medium for 24 h and stained with mouse anti-human CK 18 IgG1 and counter- stained for nuclei with DAPI (B) or rabbit anti-human AFP IgG and DAPI (C). Bar = 150 lm.

Article Snippet: For neutralizing experiments, 80% AGE-MCM was separately pre-incubated for 1 h at 37 C and 5% CO2 with mouse anti-human IL-6 IgG1 (5 lg ⁄ ml), mouse anti-human IL-1b IgG1 (5 lg ⁄ ml), rhIL-1sRI (recombinant human IL-1 soluble receptor I) (10 lg ⁄ ml), mouse anti-human TNF-a IgG1 (10 lg ⁄ ml), rhTNF-sRI (recombinant human TNF soluble receptor I) (200 ng ⁄ ml), mouse anti-human IL-6 IgG1 (5 lg ⁄ ml) + mouse anti-human IL-1b IgG1 (5 lg ⁄ ml) and mouse anti-human IL-6 IgG1 (5 lg ⁄ ml) + mouse anti-human IL-1b IgG1 (5 lg ⁄ ml) + mouse anti-human TNF-a IgG1 (10 lg ⁄ ml) (R&D Systems, Inc.) before being added to the hepatocyte culture for 48 h. Supernatants were collected and CRP levels in the supernatants were measured using the ELISA method described above.

Techniques: Staining, Cell Culture, Microscopy